Erratum in:

J Biol Chem 1995 Dec 15;270(50):30235.

J Biol Chem. 1996 May 3;271(18):11034.

Cloning and functional expression of a human eosinophil CC chemokine receptor.

Laboratory of Host Defenses, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA.

Eosinophils undergo chemotaxis, degranulate, and exhibit [C2+]i changes in response to the human CC chemokines macrophage inflammatory protein (MIP)-1 alpha, regulated on activation, normal T expressed and secreted (RANTES), and monocyte chemoattractant protein-3 (MCP-3), but the receptors involved have not been defined. We have isolated a human cDNA encoding the first eosinophil-selective chemokine receptor, designated CC chemokine receptor 3 (CC CKR3). CC CKR3 is a seven-transmembrane domain G protein-coupled receptor most closely related to the previously reported monocyte- and neutrophil-selective receptor CC CKR1 (also known as the MIP-1 alpha/RANTES receptor). When [Ca2+]i changes were monitored in stably transfected human embryonic kidney 293 cells, MIP-1 alpha and RANTES were both potent agonists for CC CKR3 and CC CKR1. However, MIP-1 beta was also an agonist for CC CKR3 but not CC CKR1; MCP-3 was an agonist for CC CKR1 but not CC CKR3. CC CKR3 may be one of the host factors responsible for selective recruitment of eosinophils to sites of inflammation.

PMID: 7622448 [PubMed - indexed for MEDLINE]