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Affinity purification of human plasma vitamin D-binding protein.
Department of Medicine, Boston University Medical Center, Massachusetts 02118, USA.
During the course of our studies to probe the vitamin D ligand-binding domains of vitamin D-binding protein and vitamin D receptor, we developed a synthetic procedure to modify the 3 beta-hydroxyl group of vitamin D3 and its 25-hydroxy- and 1,25-dihydroxy metabolites with a 3'-aminopropylether group. In the present study we have coupled 25-hydroxyvitamin D3-3 beta-3'-aminopropylether to an activated Sepharose matrix. Using this stable and reusable affinity matrix we have purified human vitamin D-binding protein from human plasma to homogeneity.
PMID: 7606167 [PubMed - indexed for MEDLINE]
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Cited by 2 PubMed Central articles
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Vitamin D binding protein-macrophage activating factor (DBP-maf) inhibits angiogenesis and tumor growth in mice.
Kisker O, Onizuka S, Becker CM, Fannon M, Flynn E, D'Amato R, Zetter B, Folkman J, Ray R, Swamy N, et al.
Neoplasia. 2003 Jan-Feb; 5(1):32-40.
[Neoplasia. 2003]
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Cubilin dysfunction causes abnormal metabolism of the steroid hormone 25(OH) vitamin D(3).
Nykjaer A, Fyfe JC, Kozyraki R, Leheste JR, Jacobsen C, Nielsen MS, Verroust PJ, Aminoff M, de la Chapelle A, Moestrup SK, et al.
Proc Natl Acad Sci U S A. 2001 Nov 20; 98(24):13895-900.
[Proc Natl Acad Sci U S A. 2001]