Abstract
Bacteriophage Mu does not grow on temperature-sensitive E. coli dnaK mutants at elevated temperatures because of a defect in late transcription. As the Mu-encoded C protein is required for activation of transcription from the phage late promoters, we attempted to determine if DnaK and its accessory proteins DnaJ and GrpE are required for synthesis of C protein or at a later step. We found that the chaperones act in Mu late transcription beyond C-protein synthesis, and that C-protein stability is decreased in the mutant hosts. This suggests that the DnaK chaperone machine may be required for the proper folding and/or multimerization of C protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / physiology
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Bacteriophage mu / genetics*
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Bacteriophage mu / growth & development
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Bacteriophage mu / metabolism
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Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
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DNA-Binding Proteins / biosynthesis*
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics
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Escherichia coli / genetics*
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Escherichia coli / physiology*
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Escherichia coli Proteins*
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Gene Expression / genetics
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HSP40 Heat-Shock Proteins
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HSP70 Heat-Shock Proteins / physiology*
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Heat-Shock Proteins / physiology
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Hot Temperature
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Kinetics
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Promoter Regions, Genetic / genetics
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Trans-Activators / biosynthesis*
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Trans-Activators / chemistry
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Trans-Activators / genetics
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Transcription, Genetic*
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Viral Proteins*
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beta-Galactosidase / genetics
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beta-Galactosidase / metabolism
Substances
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Bacterial Proteins
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Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
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C protein, Bacteriophage Mu
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DNA-Binding Proteins
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DnaJ protein, E coli
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Escherichia coli Proteins
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GrpE protein, Bacteria
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GrpE protein, E coli
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HSP40 Heat-Shock Proteins
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HSP70 Heat-Shock Proteins
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Heat-Shock Proteins
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Trans-Activators
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Viral Proteins
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beta-Galactosidase
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dnaK protein, E coli