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Ann Allergy Asthma Immunol. 1995 Oct;75(4):317-23.

Biologic potency and immunoblotting studies of extracts of three cockroach species.

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  • 1Allergic Diseases Center, University of South Alabama, Mobile, USA.

Abstract

BACKGROUND:

Although cockroaches are responsible for severe allergic disease, cockroach extracts are not yet standardized by the ID50EAL method, nor have the allergens been completely characterized.

OBJECTIVE:

To determine biologic potency and immunoblotting patterns of whole-body extracts of three cockroach species: Periplaneta americana, Blattella germanica, and Blatta orientalis.

METHOD:

Twenty-four subjects puncture test positive to mixed cockroach extract were puncture tested with the three extracts. Fourteen qualified for endpoint titration testing by the ID50EAL method to P. americana with a sum of erythema (sigma E) > or = 20 mm by puncture testing, while 13 qualified for B. germanica and 12 qualified for B. orientalis.

RESULTS:

The mean D50s were 9.67 +/- 1.33 (mean +/- SD) for P. americana, 10.24 +/- 1.16 for B. germanica, and 10.36 +/- 1.40 for B. orientalis, resulting in a potency of 1000 BAU/mL for each extract. Omitting data from subjects with puncture sigma E < 30 mm did not change the calculated potency. Sixteen serum specimens revealed up to 17 bands with molecular weight 14 to 263 kD by SDS-PAGE immunoblotting. Four specimens showed no bands; of these, one had a positive RAST only to P. americana and one only to B. germanica. There was no correlation between number of bands or RAST counts with D50.

CONCLUSIONS:

IgE immunoblots of cockroach-allergic patients vary considerably as to the number and intensity of bands. Most patients react similarly to all three species. Current nonstandardized products (1:10 wt/vol) in glycero-Coca's solution appear to contain approximately 1000 BAU/mL. Ideally these extracts should be more potent, eg, 10,000 BAU/mL.

PMID:
7583846
[PubMed - indexed for MEDLINE]
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