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Science. 1995 Oct 20;270(5235):484-7.

Serial analysis of gene expression.

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  • 1Oncology Center, Johns Hopkins University, Baltimore, MD 21231, USA.

Abstract

The characteristics of an organism are determined by the genes expressed within it. A method was developed, called serial analysis of gene expression (SAGE), that allows the quantitative and simultaneous analysis of a large number of transcripts. To demonstrate this strategy, short diagnostic sequence tags were isolated from pancreas, concatenated, and cloned. Manual sequencing of 1000 tags revealed a gene expression pattern characteristic of pancreatic function. New pancreatic transcripts corresponding to novel tags were identified. SAGE should provide a broadly applicable means for the quantitative cataloging and comparison of expressed genes in a variety of normal, developmental, and disease states.

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PMID:
7570003
[PubMed - indexed for MEDLINE]
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