Serial analysis of gene expression. [Science. 1995]

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1: Science. 1995 Oct 20;270(5235):484-7. Links

Comment in:: Science. 1995 Oct 20;270(5235):368-9, 371.

Serial analysis of gene expression.

Velculescu VE, Zhang L, Vogelstein B, Kinzler KW.

Oncology Center, Johns Hopkins University, Baltimore, MD 21231, USA.

The characteristics of an organism are determined by the genes expressed within it. A method was developed, called serial analysis of gene expression (SAGE), that allows the quantitative and simultaneous analysis of a large number of transcripts. To demonstrate this strategy, short diagnostic sequence tags were isolated from pancreas, concatenated, and cloned. Manual sequencing of 1000 tags revealed a gene expression pattern characteristic of pancreatic function. New pancreatic transcripts corresponding to novel tags were identified. SAGE should provide a broadly applicable means for the quantitative cataloging and comparison of expressed genes in a variety of normal, developmental, and disease states.

PMID: 7570003 [PubMed - indexed for MEDLINE]

Generation of longer cDNA fragments from serial analysis of gene expression tags for gene identification. [Proc Natl Acad Sci U S A. 2000]
SAGE and LongSAGE. [Methods Mol Biol. 2008]
Cloning of tissue-specific genes using serial analysis of gene expression and a novel computational substraction approach. [Genomics. 2001]
Review[Transcriptomes for serial analysis of gene expression] [J Soc Biol. 2002]
ReviewSerial analysis of gene expression: ESTs get smaller. [Bioessays. 1996]

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Serial analysis of gene expression.

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