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Proc Natl Acad Sci U S A. 1995 Sep 12;92(19):8749-53.

Rat skeletal muscle selenoprotein W: cDNA clone and mRNA modulation by dietary selenium.

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  • 1Department of Agricultural Chemistry, Oregon State University, Corvallis 97331, USA.

Abstract

Rat skeletal muscle selenoprotein W cDNA was isolated and sequenced. The isolation strategy involved design of degenerate PCR primers from reverse translation of a partial peptide sequence. A reverse transcription-coupled PCR product from rat muscle mRNA was used to screen a muscle cDNA library prepared from selenium-supplemented rats. The cDNA sequence confirmed the known protein primary sequence, including a selenocysteine residue encoded by TGA, and identified residues needed to complete the protein sequence. RNA folding algorithms predict a stem-loop structure in the 3' untranslated region of the selenoprotein W mRNA that resembles selenocysteine insertion sequence (SE-CIS) elements identified in other selenocysteine coding cDNAs. Dietary regulation of selenoprotein W mRNA was examined in rat muscle. Dietary selenium at 0.1 ppm as selenite increased muscle mRNA 4-fold relative to a selenium-deficient diet. Higher dietary selenium produced no further increase in mRNA levels.

PMID:
7568010
PMCID:
PMC41044
[PubMed - indexed for MEDLINE]
Free PMC Article
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