Abstract
We describe a DNA-specific staining procedure, using a blue platinum organic dye, which allows DNA imaging of chromosomes by detection of back-scattered electrons in the scanning electron microscope. DNA distribution is visualized within chromosomal details of the centromeric and satellite regions, or in interphase chromatin, with high-resolution (10-15 nm) for comparison with the corresponding secondary electron image representing DNA plus protein.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Centromere / genetics
-
Chlorides
-
Chromatin / genetics
-
Chromosomes / genetics*
-
Chromosomes / ultrastructure
-
Chromosomes, Human / genetics*
-
Chromosomes, Human / ultrastructure
-
DNA / analysis*
-
DNA, Plant / analysis*
-
Glutaral
-
Humans
-
Interphase
-
Metaphase
-
Microscopy, Electron, Scanning
-
Organometallic Compounds
-
Osmium Tetroxide
-
Plants / genetics*
-
Platinum Compounds
-
Staining and Labeling
Substances
-
Chlorides
-
Chromatin
-
DNA, Plant
-
Organometallic Compounds
-
Platinum Compounds
-
tetrachloroplatinate
-
uranyl acetate
-
DNA
-
Osmium Tetroxide
-
Glutaral