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Identification of a gephyrin binding motif on the glycine receptor beta subunit.
Abteilung Neurochemie, Max-Planck-Institut für Hirnforschung, Frankfurt Federal Republic of Germany.
The tubulin-binding protein gephyrin copurifies with the inhibitory glycine receptor (GlyR) and is essential for its postsynaptic localization. Here we have analyzed the interaction between the GlyR and recombinant gephyrin and identified a gephyrin binding site in the cytoplasmic loop between the third and fourth transmembrane segments of the beta subunit. GlyR alpha subunits and GABAA receptor proteins failed to bind recombinant gephyrin. However, insertion of an 18 residue segment of the GlyR beta subunit into the GABAA receptor beta 1 subunit conferred gephyrin binding both in an overlay assay and in transfected mammalian cells. These results indicate that beta subunit expression is essential for the formation of a postsynaptic GlyR matrix.
PMID: 7546736 [PubMed - indexed for MEDLINE]
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Cited by 28 PubMed Central articles
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Synaptic activation modifies microtubules underlying transport of postsynaptic cargo.
Maas C, Belgardt D, Lee HK, Heisler FF, Lappe-Siefke C, Magiera MM, van Dijk J, Hausrat TJ, Janke C, Kneussel M.
Proc Natl Acad Sci U S A. 2009 May 26; 106(21):8731-6. Epub 2009 May 13.
[Proc Natl Acad Sci U S A. 2009]
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Extrasynaptic and postsynaptic receptors in glycinergic and GABAergic neurotransmission: a division of labor?
Muller E, Le-Corronc H, Legendre P.
Front Mol Neurosci. 2008; 1:3. Epub 2008 Mar 28.
[Front Mol Neurosci. 2008]
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Impaired GABAergic transmission and altered hippocampal synaptic plasticity in collybistin-deficient mice.
Papadopoulos T, Korte M, Eulenburg V, Kubota H, Retiounskaia M, Harvey RJ, Harvey K, O'Sullivan GA, Laube B, Hülsmann S, et al.
EMBO J. 2007 Sep 5; 26(17):3888-99. Epub 2007 Aug 9.
[EMBO J. 2007]
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