Diagnostic assay for Helicobacter hepaticus based on nucleotide sequence of its 16S rRNA gene

J K Battles; J C Williamson; K M Pike; P L Gorelick; J M Ward; M A Gonda

doi:10.1128/jcm.33.5.1344-1347.1995

Diagnostic assay for Helicobacter hepaticus based on nucleotide sequence of its 16S rRNA gene

J Clin Microbiol. 1995 May;33(5):1344-7. doi: 10.1128/jcm.33.5.1344-1347.1995.

Authors

J K Battles¹, J C Williamson, K M Pike, P L Gorelick, J M Ward, M A Gonda

Affiliation

¹ Laboratory of Cell and Molecular Structure, Program Resources, Inc./DynCorp, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702-1201, USA.

Abstract

Conserved primers were used to PCR amplify 95% of the Helicobacter hepaticus 16S rRNA gene. Its sequence was determined and aligned to those of related bacteria, enabling the selection of primers to highly diverged regions of the 16S rRNA gene and an oligonucleotide probe for the development of a PCR-liquid hybridization assay. This assay was shown to be both sensitive and specific for H. hepaticus 16S rRNA gene sequences.

MeSH terms

Animals
Bacteriological Techniques / statistics & numerical data
Base Sequence
Conserved Sequence
DNA Primers / genetics
DNA, Bacterial / genetics
DNA, Ribosomal / genetics
Genes, Bacterial*
Helicobacter / genetics*
Helicobacter / isolation & purification
Helicobacter Infections / diagnosis
Helicobacter Infections / microbiology
Humans
Male
Mice
Mice, SCID
Molecular Sequence Data
Nucleic Acid Hybridization
Polymerase Chain Reaction / methods
Polymerase Chain Reaction / statistics & numerical data
RNA, Bacterial / genetics*
RNA, Ribosomal, 16S / genetics*
Sensitivity and Specificity

Substances

DNA Primers
DNA, Bacterial
DNA, Ribosomal
RNA, Bacterial
RNA, Ribosomal, 16S

Associated data

GENBANK/L39122