Histochemistry. 1994 Dec;102(6):483-6.

Lectin staining of sheep microglia.

Source

Department of Neuroscience, JHMHC, University of Florida, Gainesville 32610.

Abstract

The B4-isolectin from Griffonia simplicifolia is known to stain microglial cells in a variety of species. The present report describes a lectin staining method that has been modified to facilitate staining of resting microglia, as well as perivascular cells, in vibratome sections of normal sheep brain. This modified method employs tissue fixed in formaldehyde or paraformaldehyde and requires incubating sections with Triton X-100 prior to staining.

PMID:: 7535300; [PubMed - indexed for MEDLINE]

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

OhioLINK Electronic Journal Center

Other Literature Sources

COS Scholar Universe

Supplemental Content

Related citations

Effects of trypsinization and microwave treatment on lectin labelling of microglial cells in paraffin-embedded sections from pre- and postnatal bovine brains. [Acta Histochem. 1995]
Effects of trypsinization and microwave treatment on lectin labelling of microglial cells in paraffin-embedded sections from pre- and postnatal bovine brains.
Hewicker-Trautwein M, Schultheis G, Trautwein G. Acta Histochem. 1995 Oct; 97(4):455-61.
Lectin labelling of amoeboid and ramified microglial cells in the telencephalon of ovine fetuses with the B4 isolectin from Griffonia simplicifolia. [J Comp Pathol. 1994]
Lectin labelling of amoeboid and ramified microglial cells in the telencephalon of ovine fetuses with the B4 isolectin from Griffonia simplicifolia.
Hewicker-Trautwein M, Schultheis G. J Comp Pathol. 1994 Jul; 111(1):21-31.
An improved staining method for rat microglial cells using the lectin from Griffonia simplicifolia (GSA I-B4). [J Histochem Cytochem. 1990]
An improved staining method for rat microglial cells using the lectin from Griffonia simplicifolia (GSA I-B4).
Streit WJ. J Histochem Cytochem. 1990 Nov; 38(11):1683-6.
Review Proliferation of differentiated glial cells in the brain stem. [Braz J Med Biol Res. 1998]
Review Proliferation of differentiated glial cells in the brain stem.
Barradas PC, Cavalcante LA. Braz J Med Biol Res. 1998 Feb; 31(2):257-70.
Review [Lectin staining of tissues and cells]. [Tanpakushitsu Kakusan Koso. 1992]
Review [Lectin staining of tissues and cells].
Takata K, Kawakami H, Hirano H, Nishiyama F. Tanpakushitsu Kakusan Koso. 1992 Aug; 37(11 Suppl):2018-25.

See reviews... See all...

Cited by 2 PubMed Central articles

Fibrillar beta-amyloid peptide Abeta1-40 activates microglial proliferation via stimulating TNF-alpha release and H2O2 derived from NADPH oxidase: a cell culture study. [J Neuroinflammation. 2006]
Fibrillar beta-amyloid peptide Abeta1-40 activates microglial proliferation via stimulating TNF-alpha release and H2O2 derived from NADPH oxidase: a cell culture study.
Jekabsone A, Mander PK, Tickler A, Sharpe M, Brown GC. J Neuroinflammation. 2006 Sep 7; 3:24. Epub 2006 Sep 7.
Injectable self-assembling peptide nanofibers create intramyocardial microenvironments for endothelial cells. [Circulation. 2005]
Injectable self-assembling peptide nanofibers create intramyocardial microenvironments for endothelial cells.
Davis ME, Motion JP, Narmoneva DA, Takahashi T, Hakuno D, Kamm RD, Zhang S, Lee RT. Circulation. 2005 Feb 1; 111(4):442-50.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Lectin staining of sheep microglia.
Lectin staining of sheep microglia.
Histochemistry. 1994 Dec ;102(6):483-6.

PubMed
Oxidative stress during viral infection: a review.
Oxidative stress during viral infection: a review.
Free Radic Biol Med. 1996 ;21(5):641-9.

PubMed
High risk of HIV-related mortality is associated with selenium deficiency.
High risk of HIV-related mortality is associated with selenium deficiency.
J Acquir Immune Defic Syndr Hum Retrovirol. 1997 Aug 15 ;15(5):370-4.

PubMed
Anal squamous intraepithelial lesions in HIV-positive and HIV-negative homosexua...
Anal squamous intraepithelial lesions in HIV-positive and HIV-negative homosexual and bisexual men: prevalence and risk factors.
J Acquir Immune Defic Syndr Hum Retrovirol. 1998 Apr 1 ;17(4):320-6.

PubMed
Smoking and risk of dementia and Alzheimer's disease in a population-based cohor...
Smoking and risk of dementia and Alzheimer's disease in a population-based cohort study: the Rotterdam Study.
Lancet. 1998 Jun 20 ;351(9119):1840-3.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to: