Abstract
A modified hepatitis B virus (HBV) surface antigen, the SA-28 protein, was constructed and expressed by recombinant vaccinia virus in mammalian cells. This protein was composed of a PreS1 region-derived peptide (amino acids 21 to 47) that contained the hepatocyte receptor-binding site, joined to the C terminus of the major S protein at amino acid position 223. This modified surface antigen could be efficiently assembled into particles with a density of 1.23 g/ml and could be secreted from several mammalian cell lines. The results of immunoprecipitation revealed that the SA-28 protein was recognized by both the anti-S protein antibody and the anti-PreS1 antibody. A strong antibody response, against both the S protein and PreS1 epitopes, was induced in BALB/c mice immunized by the SA-28 particles indicating good immunogenicity. These results suggested that the HBV surface antigen consisting of the SA-28 protein could be a promising candidate as a new HBV vaccine with higher efficacy.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigen-Antibody Reactions
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Binding Sites
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Cell Line
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Epitopes / immunology
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Gene Expression
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Hepatitis Antibodies / blood
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Hepatitis B Surface Antigens / biosynthesis
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Hepatitis B Surface Antigens / chemistry
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Hepatitis B Surface Antigens / genetics
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Hepatitis B Surface Antigens / immunology*
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Hepatitis B virus / genetics
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Hepatitis B virus / immunology
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Liver / cytology
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Liver / virology
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Mice
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Mice, Inbred BALB C
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Protein Precursors / biosynthesis
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Protein Precursors / chemistry
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Protein Precursors / genetics
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Protein Precursors / immunology*
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Receptors, Antigen / metabolism*
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Receptors, Virus / metabolism*
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / immunology*
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Vaccination
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Vaccinia virus / genetics
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / immunology*
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Viral Vaccines
Substances
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Epitopes
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Hepatitis Antibodies
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Hepatitis B Surface Antigens
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Protein Precursors
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Receptors, Antigen
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Receptors, Virus
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Recombinant Fusion Proteins
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Viral Envelope Proteins
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Viral Vaccines
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hepatitis B surface antigen receptor
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presurface protein 1, hepatitis B surface antigen