Differences in antigenicity of E2 in Semliki Forest virus particles and in infected cells

S Repges-Illguth; G Kaluza

doi:10.1007/BF01310027

Differences in antigenicity of E2 in Semliki Forest virus particles and in infected cells

Arch Virol. 1994;135(3-4):433-5. doi: 10.1007/BF01310027.

Authors

S Repges-Illguth¹, G Kaluza

Affiliation

¹ Institute of Medical Virology, Justus-Leibig University Giessen, Federal Republic of Germany.

Abstract

Using six monoclonal antibodies to epitopes a-f on the glycoprotein E2 of Semliki Forest virus (SFV) we found antigenic differences between E2 in infected cells and in virus particles, respectively, if glycosylation was impaired by 2-deoxy-D-glucose or inhibited by N-methyl-1-deoxynojirimycin. Furthermore we concluded that a conformational change of E2 takes place on virus budding.

Publication types

Comparative Study

MeSH terms

1-Deoxynojirimycin / analogs & derivatives
1-Deoxynojirimycin / pharmacology
Animals
Antibodies, Monoclonal
Antigens, Viral / analysis*
Antigens, Viral / biosynthesis
Antigens, Viral / immunology
Antiviral Agents / pharmacology
Cells, Cultured
Chick Embryo
Deoxyglucose / pharmacology
Epitopes / analysis*
Glycosylation / drug effects
Protein Conformation
Semliki forest virus / drug effects
Semliki forest virus / immunology*
Semliki forest virus / metabolism
Viral Envelope Proteins / chemistry
Viral Envelope Proteins / immunology
Viral Envelope Proteins / metabolism*

Substances

Antibodies, Monoclonal
Antigens, Viral
Antiviral Agents
Epitopes
Viral Envelope Proteins
1-Deoxynojirimycin
Deoxyglucose
N-methyldeoxynojirimycin