Vaccine. 1994 Jul;12(9):825-32.

Characterization of binding and TNF-alpha-inducing ability of chitosans on monocytes: the involvement of CD14.

Otterlei M, Vårum KM, Ryan L, Espevik T.

Institute of Cancer Research, University of Trondheim, University Medical Centre (MTS), Norway.

Chitosans with different chemical composition were found to induce TNF-alpha production from human monocytes. Their ability to induce TNF-alpha was found to be highly dependent on neutral-solubility and molecular weight. Monoclonal antibodies against CD14 inhibited TNF-alpha production from monocytes stimulated with neutral-soluble chitosans. Binding studies indicated that lipopolysaccharides (LPS) and neutral-soluble chitosans share a binding site on monocytes which involves CD14. TNF-alpha production from monocytes stimulated with chitosans was dependent on serum. LPS-binding protein (LBP) enhanced the chitosan-induced TNF-alpha production only to a minor degree, suggesting that serum proteins other than LBP play an important role in the stimulatory effect.

PMID: 7526573 [PubMed - indexed for MEDLINE]

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Influence of CD14, LBP and BPI in the monocyte response to LPS of different polysaccharide chain length.
Scand J Immunol. 1995 Jul; 42(1):119-27.

[Scand J Immunol. 1995]
CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein.
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[Science. 1990]
Membrane molecules which trigger the production of interleukin-1 and tumor necrosis factor-alpha by lipopolysaccharide-stimulated human monocytes.
Eur J Immunol. 1992 Jun; 22(6):1461-6.

[Eur J Immunol. 1992]
ReviewLipopolysaccharide binding protein and CD14 in LPS dependent macrophage activation.
Immunobiology. 1993 Apr; 187(3-5):227-32.

[Immunobiology. 1993]
ReviewCD14 and other recognition molecules for lipopolysaccharide: a review.
Immunopharmacology. 1995 Apr; 29(3):187-205.

[Immunopharmacology. 1995]
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Characterization of binding and TNF-alpha-inducing ability of chitosans on monocytes: the involvement of CD14.

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