Display Settings:


Send to:

Choose Destination
See comment in PubMed Commons below
Science. 1994 Sep 30;265(5181):2085-8.

Padlock probes: circularizing oligonucleotides for localized DNA detection.

Author information

  • 1Beijer Laboratory, Department of Medical Genetics, Biomedical Center, Uppsala, Sweden.


Nucleotide sequence information derived from DNA segments of the human and other genomes is accumulating rapidly. However, it frequently proves difficult to use such short DNA segments to identify clones in genomic libraries or fragments in blots of the whole genome or for in situ analysis of chromosomes. Oligonucleotide probes, consisting of two target-complementary segments, connected by a linker sequence, were designed. Upon recognition of the specific nucleic acid molecule the ends of the probes were joined through the action of a ligase, creating circular DNA molecules catenated to the target sequence. These probes thus provide highly specific detection with minimal background.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire
    Loading ...
    Write to the Help Desk