Nucleotide sequence and expression of a ripening and water stress-related cDNA from tomato with homology to the MIP class of membrane channel proteins

R G Fray; A Wallace; D Grierson; G W Lycett

doi:10.1007/BF00024122

Nucleotide sequence and expression of a ripening and water stress-related cDNA from tomato with homology to the MIP class of membrane channel proteins

Plant Mol Biol. 1994 Feb;24(3):539-43. doi: 10.1007/BF00024122.

Authors

R G Fray¹, A Wallace, D Grierson, G W Lycett

Affiliation

¹ AFRC Research Group in Plant Gene Regulation, University of Nottingham, Loughborough, UK.

PMID: 7510135
DOI: 10.1007/BF00024122

Abstract

The nucleotide sequence and derived amino acid sequence were determined for a full-length version of the tomato cDNA clone, pTOM75, the mRNA for which has previously been shown to accumulate in roots, ripening fruit and senescing leaves. Computer analysis of the predicted protein product, which we have named tomato ripening-associated membrane protein (TRAMP) indicates strong homology to known transmembrane channel proteins from other organisms. Northern analysis showed that this gene was induced by waterstress and that this induction was unaffected in an ABA-deficient genetic background.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Bacterial Proteins / genetics*
Base Sequence
Blotting, Northern
DNA, Complementary / biosynthesis
DNA, Complementary / genetics*
Gene Expression
Immunophilins*
Membrane Proteins / biosynthesis
Membrane Proteins / genetics*
Molecular Sequence Data
Peptidylprolyl Isomerase*
Plant Proteins*
RNA / analysis
RNA / biosynthesis
Sequence Homology, Amino Acid
Vegetables / genetics*

Substances

Bacterial Proteins
DNA, Complementary
Membrane Proteins
Plant Proteins
TRAMP protein, Lycopersicon esculentum
RNA
Immunophilins
Peptidylprolyl Isomerase

Associated data

GENBANK/X73847
GENBANK/X73848