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Cloning and expression of a human glucagon receptor.
Department of Molecular Pharmacology and Biochemistry, Merck Research Laboratories, Rahway, NJ 07065.
A human glucagon receptor has been cloned from human liver tissue. The 1578-bp cDNA clone encodes a protein of 477 amino acids with 82% identity to the rat glucagon receptor. The predicted secondary structure and homology to known proteins places this receptor within the superfamily of seven transmembrane domain G protein coupled receptors. Transfection of the human glucagon receptor into COS-7 cells confers upon them high affinity binding for [125I] glucagon. In membranes prepared from COS-7 cells transfected with the human glucagon receptor, the binding of [125I] glucagon is inhibited with the rank order of potency glucagon > oxyntomodulin > glucagon-like peptide 1 (7-36) amide >> glucagon-like peptide 2 = gastric inhibitory peptide = secretin.
PMID: 7507321 [PubMed - indexed for MEDLINE]
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Cited by 4 PubMed Central articles
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Identification and characterization of a second melanin-concentrating hormone receptor, MCH-2R.
Sailer AW, Sano H, Zeng Z, McDonald TP, Pan J, Pong SS, Feighner SD, Tan CP, Fukami T, Iwaasa H, et al.
Proc Natl Acad Sci U S A. 2001 Jun 19; 98(13):7564-9. Epub 2001 Jun 12.
[Proc Natl Acad Sci U S A. 2001]
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Prototypic G protein-coupled receptor for the intestinotrophic factor glucagon-like peptide 2.
Munroe DG, Gupta AK, Kooshesh F, Vyas TB, Rizkalla G, Wang H, Demchyshyn L, Yang ZJ, Kamboj RK, Chen H, et al.
Proc Natl Acad Sci U S A. 1999 Feb 16; 96(4):1569-73.
[Proc Natl Acad Sci U S A. 1999]
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Co-transfection with protein kinase D confers phorbol-ester-mediated inhibition on glucagon-stimulated cAMP accumulation in COS cells transfected to overexpress glucagon receptors.
Tobias ES, Rozengurt E, Connell JM, Houslay MD.
Biochem J. 1997 Sep 1; 326 ( Pt 2):545-51.
[Biochem J. 1997]
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