Nat Med. 1995 Dec;1(12):1284-90.

In vivo fate of HIV-1-infected T cells: quantitative analysis of the transition to stable latency.

Chun TW, Finzi D, Margolick J, Chadwick K, Schwartz D, Siliciano RF.

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Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

Abstract

Although it is presumed that the integration of HIV-1 into the genome of infected CD4+ T lymphocytes allows viral persistence, there has been little direct evidence that CD4+ T cells with integrated provirus function as a latent reservoir for HIV-1 in infected individuals. Using resting CD4+ T-cell populations of extremely high purity and a novel assay that selectively and unambiguously detects integrated HIV-1, we show that resting CD4+ T cells harbouring integrated provirus are present in some infected individuals. However, these cells do not accumulate within the circulating pool of resting CD4+ T cells in the early stages of HIV-1 infection and do not accumulate even after prolonged periods in long-term survivors of HIV-1 infection. These results suggest that because of viral cytopathic effects and/or host effector mechanisms, productively infected CD4+ T cells do not generally survive for long enough to revert to a resting memory state in vivo.

PMID:: 7489410; [PubMed - indexed for MEDLINE]

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In vivo fate of HIV-1-infected T cells: quantitative analysis of the transition to stable latency.

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