Abstract
We examined effects of an isoquinolinesulfonamide derivative, KN-62, on human ovarian cancer cells, NOS3AR, that are resistant to Adriamycin (ADR). MTT assay revealed that 10 microM KN-62 overcame the resistance. KN-62 had little effect on GST activity. In studies on the intracellular accumulation of ADR, KN-62 increased the ADR contents in the resistant cells close to the level seen in the sensitive cells. These results suggest that the reversal of the resistance against ADR in ovarian cancer cells by KN-62 is mainly due to higher accumulation of ADR in NOS3AR cells. Furthermore, we detected Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) in NOS3AR cells since KN-62 is a specific inhibitor of the kinase. In this paper, we discussed on modulation of ADR-resistance by KN-62.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine* / analogs & derivatives*
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / analysis
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / biosynthesis*
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / isolation & purification
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Blotting, Western
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Calcium-Calmodulin-Dependent Protein Kinase Type 2
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Calcium-Calmodulin-Dependent Protein Kinases / antagonists & inhibitors*
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Cell Line
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Cell Survival / drug effects
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Cystadenocarcinoma
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Dose-Response Relationship, Drug
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Doxorubicin / toxicity*
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Drug Resistance, Neoplasm*
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Electrophoresis, Polyacrylamide Gel
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Enzyme Inhibitors / pharmacology*
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Female
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Glutathione Transferase / metabolism*
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Humans
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Isoquinolines / pharmacology*
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Kinetics
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Ovarian Neoplasms
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Piperazines / pharmacology*
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Tumor Cells, Cultured
Substances
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Enzyme Inhibitors
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Isoquinolines
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Piperazines
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KN 62
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Doxorubicin
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
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Glutathione Transferase
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Calcium-Calmodulin-Dependent Protein Kinase Type 2
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Calcium-Calmodulin-Dependent Protein Kinases