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Appl Environ Microbiol. 1995 Aug;61(8):3169-71.

Cloning, nucleotide sequencing, and expression of an opine dehydrogenase gene from Arthrobacter sp. strain 1C.

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  • 1Biotechnology Research Center, Toyama Prefectural University, Japan.

Abstract

The gene coding for opine dehydrogenase from Arthrobacter sp. strain 1C was cloned onto plasmid pBluescript KS(-), and the nucleotide sequence of the 1,077-bp open reading frame consisting of 359 codons was identified as the odh gene. Transformed Escherichia coli cells overproduced NAD+-dependent opine dehydrogenase under control of the promoter of the lac gene on pBluescript KS(-).

PMID:
7487048
[PubMed - indexed for MEDLINE]
PMCID:
PMC167592
Free PMC Article
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