Abstract
A species-specific PCR for the identification of Naegleria fowleri was developed. In sensitivity studies, 10 trophozoites or cysts and 1 trophozoite or cyst could be detected after 35 and 45 cycles, respectively. In conjunction with a rapid DNA isolation method, this PCR was used to identify N. fowleri directly from primary cultures of environmental samples.
MeSH terms
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Animals
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Base Sequence
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DNA Primers / genetics
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DNA, Protozoan / genetics
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Humans
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Molecular Sequence Data
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Naegleria fowleri / genetics*
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Naegleria fowleri / isolation & purification*
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Polymerase Chain Reaction / methods*
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Polymerase Chain Reaction / statistics & numerical data
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Polymorphism, Restriction Fragment Length
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Sensitivity and Specificity
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Species Specificity
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Water / parasitology
Substances
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DNA Primers
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DNA, Protozoan
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Water