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    Anal Biochem. 1995 Aug 10;229(2):313-6.

    Chromogenic assay for phospholipase C from Bacillus cereus.

    Hergenrother PJ, Spaller MR, Haas MK, Martin SF.

    Department of Chemistry and Biochemistry, University of Texas, Austin 78712, USA.

    A standard chromogenic assay for phosphatidylcholine determination has been modified in the development of a facile method for measuring the rate parameters (Km and Vmax) for phosphatidylcholine-preferring phospholipase C from Bacillus cereus (PLCBc). The assay is applied to the determination of the Ki for a known inhibitor of PLCBc.

    PMID: 7485988 [PubMed - indexed for MEDLINE]

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