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J Biol Chem. 1981 Dec 10;256(23):12058-67.

Structural gene products of the Ah locus. Evidence for many unique P-450-mediated monooxygenase activities reconstituted from 3-methylcholanthrene-treated C57BL/6N mouse liver microsomes.

Abstract

Cytochrome P-450 from cholate-solubilized liver microsomes prepared from 3-methylcholanthrene-treated genetically "responsive" C57BL/6N mice (Ahb/Ahb) was partially purified by aminooctyl-Sepharose 4B column chromatography with an elution buffer that included Emulgen 911, followed by hydroxylapatite column chromatography. The P-450 was separated into 16 fractions: 15 fractions principally associated with the Ahb allele and induction by 3-methylcholanthrene and 1 fraction not associated with the Ahb allele, i.e. predominantly constitutive form(s) of P-450. The metabolism of benzo[a]pyrene, 7-ethoxycoumarin, biphenyl, ethoxyresorufin, acetanilide, 2-acetylaminofluorene, phenacetin, estradiol-17 beta, and testosterone was investigated. With these nine substrates, we examined the rates of formation of 17 products, i.e. 17 P-450-mediated reconstituted monooxygenase "'activities," in each of the 16 fractions. Differing reductase/cytochrome and lipid/cytochrome requirements were observed for each activity; the reasons for these empirical differences are not known. By two-factor analysis of variance our data for 17 catalytic activities can be explained by a minimum of 19 unique groups of monooxygenase activities: 12 induced by 3-methylcholanthrene and 7 control (endogenous). At least one of these distinctly different 3-methylcholanthrene-induced groups and at least one of these constitutive groups is associated with a marked blue spectral shift (approximately 2.0 nm) in the Soret peak of the reduced hemoprotein.CO complex, suggesting multiple forms of inducible and control "P-448." Almost every reconstituted monooxygenase activity therefore appears to be unique and probably represents the aggregate activity from numerous forms of P-450.

PMID:
7298644
[PubMed - indexed for MEDLINE]
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