An electrophoretic examination is made of mild samples taken from eight Bali (banteng) cattle, Bos (bibos) javanicus, at Beatrice Hills, Northern territory, Australia. Starch-gel electrophoresis at pH 9.5 (NaOH-H3BO3 buffer) and filter-paper electrophoresis at pH 8.6 (diethylbarbiturate buffer) indicate that all samples contain a new alpha-lactalbumin variant, designated alpha-lactalbumin C. The order of mobility for bovine variants is / greater than B greater than C. The C variant differs from the common B variant in having one more amide residue (substitution or Gln for Glu). Examination of milk samples by urea-starch-gel electrophoresis at alkaline pH indicates that there is a new alpha S1-casein variant, designated alpha S1-casein EBali, present in some samples. No new kappa-casein variant is detected by this method (all samples typing as kappa-casein B). A new variant of beta-casein, designated A4, is detected by urea-starch-gel electrophoresis at low pH. The variants of milk proteins observed in this paper and in Bell et al. (1981) are discussed in relation to those of other members of the Bovinae, especially the yak, bos (Poephagus) grunniens.