Biochim Biophys Acta. 1981 May 14;659(1):169-78.

Purification and partial characterization of prostaglandin D2 11-keto reductase in rabbit liver.

Abstract

The cytoplasmic NADPH-dependent prostaglandin D2 11-keto reductase from rabbit liver was purified by a series of chromatographic procedures including isoelectric focusing. The enzyme catalyzed the conversion of prostaglandin D2 to prostaglandin F2 alpha and had a pH optimum of 7.0-7.5, and an isoelectric point of 5.8. The molecular weight was estimated to be 66 000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Enzymic activity was time and concentration dependent and required NADPH as cofactor.

PMID:: 7248317; [PubMed - indexed for MEDLINE]

Supplemental Content

Save items

Related citations in PubMed

A novel prostaglandin 11-keto reductase found in rabbit liver. [Biochim Biophys Acta. 1981]
A novel prostaglandin 11-keto reductase found in rabbit liver.
Reingold DF, Kawasaki A, Needleman P. Biochim Biophys Acta. 1981 May 14; 659(1):179-88.
Metabolism of prostacyclin by 9-hydroxyprostaglandin dehydrogenase in human platelets. Formation of a potent inhibitor of platelet aggregation and enzyme purification. [J Biol Chem. 1980]
Metabolism of prostacyclin by 9-hydroxyprostaglandin dehydrogenase in human platelets. Formation of a potent inhibitor of platelet aggregation and enzyme purification.
Wong PY, Lee WH, Chao PH, Reiss RF, McGiff JC. J Biol Chem. 1980 Oct 10; 255(19):9021-4.
Purification of PGD2 11-ketoreductase from rabbit liver. [Methods Enzymol. 1982]
Purification of PGD2 11-ketoreductase from rabbit liver.
Wong PY. Methods Enzymol. 1982; 86:117-25.
Review Formation and metabolism of prostaglandins in the kidney. [Kidney Int. 1981]
Review Formation and metabolism of prostaglandins in the kidney.
Anggård E, Oliw E. Kidney Int. 1981 Jun; 19(6):771-80.
Review Prostaglandin F synthase. [Prostaglandins Other Lipid Med...]
Review Prostaglandin F synthase.
Watanabe K. Prostaglandins Other Lipid Mediat. 2002 Aug; 68-69:401-7.

See reviews... See all...

Cited by 7 PubMed Central articles

9-Deoxy-delta 9, delta 12-13,14-dihydroprostaglandin D2, a metabolite of prostaglandin D2 formed in human plasma. [Proc Natl Acad Sci U S A. 1984]
9-Deoxy-delta 9, delta 12-13,14-dihydroprostaglandin D2, a metabolite of prostaglandin D2 formed in human plasma.
Kikawa Y, Narumiya S, Fukushima M, Wakatsuka H, Hayaishi O. Proc Natl Acad Sci U S A. 1984 Mar; 81(5):1317-21.
Transformation of prostaglandin D2 to 9 alpha, 11 beta-(15S)-trihydroxyprosta-(5Z,13E)-dien-1-oic acid (9 alpha, 11 beta-prostaglandin F2): a unique biologically active prostaglandin produced enzymatically in vivo in humans. [Proc Natl Acad Sci U S A. 1985]
Transformation of prostaglandin D2 to 9 alpha, 11 beta-(15S)-trihydroxyprosta-(5Z,13E)-dien-1-oic acid (9 alpha, 11 beta-prostaglandin F2): a unique biologically active prostaglandin produced enzymatically in vivo in humans.
Liston TE, Roberts LJ 2nd. Proc Natl Acad Sci U S A. 1985 Sep; 82(18):6030-4.
Evidence against the formation of 13,14-dihydro-15-keto-prostaglandin F2 alpha following inhalation of prostaglandin D2 in man. [Br J Pharmacol. 1986]
Evidence against the formation of 13,14-dihydro-15-keto-prostaglandin F2 alpha following inhalation of prostaglandin D2 in man.
Hardy CC, Holgate ST, Robinson C. Br J Pharmacol. 1986 Mar; 87(3):563-8.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Purification and partial characterization of prostaglandin D2 11-keto reductase ...
Purification and partial characterization of prostaglandin D2 11-keto reductase in rabbit liver.
Biochim Biophys Acta. 1981 May 14 ;659(1):169-78.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Purification and partial characterization of prostaglandin D2 11-keto reductase in rabbit liver.

Abstract

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

Supplemental Content

Save items

Related citations in PubMed

Cited by 7 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information