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    Clin Chim Acta. 1978 Dec 1;90(2):121-7.

    Improvement of the FeCl3 test for phenylpyruvic acid.

    Cassidei L, Dell'atti A, Sciacovelli O.

    Abstract

    A simple alternative colorimetric method has been developed for quantitative determination of phenylpyruvic acid in urine based on the improved stability of the ferric complex of enol-phenylpyruvate in water-dimethylsulfoxide 50% vol. mixed solvent. The method can be applied directly to urine or to a dry ether-extract of urine. Operating on the dry ether-extract of urine, detection of phenylpyruvic acid is possible at an amount of as low as 1 mg/100 ml. Whereas, if urine samples are processed, detection is possible for a concentration of as low as 5 mg/100 ml and quantitative determination for a concentration greater than 10 mg/100 ml. The test carried out on a diaper wet with urine retains the same sensitivity and shows instantaneous development of the green colour.

    PMID:
    719897
    [PubMed - indexed for MEDLINE]

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