Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Anat Embryol (Berl). 1982 Dec;165(3):405-13.

Isolated osteoclasts in primary culture: first observations on structure and survival in culture media.

Abstract

Osteoclasts were isolated mechanically from the medullary bone of laying hens kept 7 days on a low calcium diet. Osteoclast enrichment was achieved with 3-4 sedimentations of the cell suspension in test-tubes prepared by layering on the bottom with BSA 10% in MEM-HEPES or PBS, above which the cells were suspended in MEM-HEPES or PBS. The final suspension of osteoclasts was cultivated in MEM with 10% FCS for 3 weeks. The cultures were observed by phase-contrast and scanning electron microscopy (SEM). By the third day, the osteoclasts were completely spread onto the plastic dishes and a variety of morphologies and of intercellular contacts was established. Osteoclasts in culture do not lose their morphology; they survive for long periods and can be used in many experimental systems.

PMID:
7158821
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk