In vitro vinblastine-induced tubulin paracrystals

J Biol Chem. 1982 Sep 10;257(17):10387-91.

Abstract

Purified calf brain tubulin has been induced to self-aggregate in vitro into paracrystalline structures by the anti-cancer drug vinblastine. The size, shape, optical birefringence, and drug stoichiometry of these tubulin-vinblastine paracrystals are similar to those of paracrystals formed in vivo. Similar structures can be formed with vincristine and desacetylvinblastine, but not with colchicine or podophyllotoxin.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Brain Chemistry
  • Cattle
  • Colchicine
  • Crystallization
  • Kinetics
  • Podophyllotoxin
  • Structure-Activity Relationship
  • Tubulin* / isolation & purification
  • Vinblastine* / analogs & derivatives

Substances

  • Tubulin
  • Vinblastine
  • Podophyllotoxin
  • Colchicine