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    Jikken Dobutsu. 1978 Jul;27(3):271-81.

    [Establishment of a new breeding colony of germfree CF no. 1 mice (author's transl)].

    [Article in Japanese]

    Abstract

    Baby CF no. 1 mice were obtained by hysterectomy and reared by hand feeding under aseptic condition. Temperature in the cage was kept at 33 degrees C for first 14 days of age. Artificial milk, diet RM was composed of rat milk (44%) collected from the stomach of killed baby rats, evaporated cow milk (16%) and vitamin mixture (2%). The diet was homogenized after sterilization and given mice using a latex gum stomach tube at intervals of four hours for 20 to 24 days of age. Feeding size a day (Y ml) was given by the following regression curve, Y = 0.412 X - 0.299, (X : body weights in gram). According to this procedure, 40 out of 74 germfree mice (54%) were weaned with good growth. From a male and a female mice, the germfree CF no. 1 mouse colony of Takeda was established in 1967 and the lines were distributed to several laboratories of this country.

    PMID:
    710515
    [PubMed - indexed for MEDLINE]

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