Radioimmunoassay for the detection of antigen-specific IgM, IgG, and IgA in equine sera

Am J Vet Res. 1982 Feb;43(2):294-8.

Abstract

A radioimmunoassay was developed to discriminate immunoglobulin (Ig) classes specific for the J-5 mutant of Escherichia coli (serotype O:111-B4). Adult horses were periodically inoculated IM with a nonviable suspension of the J-5 mutant emulsified in Freund's incomplete adjuvant. Before and after the horses were inoculated, sera were collected sequentially and examined by radioimmunoassay. Rabbit anti-(horse) Ig and [125I]protein A served as the indicator system. Antigen-specific IgM, IgG, and IgA were observed to follow a classic immune response. The radioimmunoassay offers a valuable tool for the discrimination of circulating, antigen-specific Ig in the horse.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Bacterial / analysis*
  • Bacterial Vaccines / immunology
  • Escherichia coli / genetics
  • Escherichia coli / immunology
  • Horses / immunology*
  • Immunoglobulin A / analysis*
  • Immunoglobulin G / analysis*
  • Immunoglobulin M / analysis*
  • Mutation
  • Radioimmunoassay / veterinary*
  • Vaccination / veterinary

Substances

  • Antibodies, Bacterial
  • Bacterial Vaccines
  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin M