Abstract
We have cloned and characterized a G+C-rich 2.2-kilobase pair Bam HI fragment which contains a single Syrian hamster 5 S rRNA gene. The cloned fragment directs the synthesis in vitro of 5 S RNA catalyzed by RNA polymerase III. Southern hybridization analysis of genomic hamster DNA indicates that the sequences present in the clone are tandemly repeated with a redundancy of 2700 copies per diploid cell.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Cell Line
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Cricetinae
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Cricetulus
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DNA / metabolism*
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Female
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Genes*
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Kidney
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Nucleic Acid Hybridization
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Oocytes / metabolism
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Plasmids
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Protein Biosynthesis
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RNA Polymerase III / metabolism
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RNA, Ribosomal / genetics*
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Transcription, Genetic
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Xenopus
Substances
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RNA, Ribosomal
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DNA
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RNA Polymerase III