Structure and organization of a mammalian 5 S gene cluster

J Biol Chem. 1982 Oct 10;257(19):11706-11.

Abstract

We have cloned and characterized a G+C-rich 2.2-kilobase pair Bam HI fragment which contains a single Syrian hamster 5 S rRNA gene. The cloned fragment directs the synthesis in vitro of 5 S RNA catalyzed by RNA polymerase III. Southern hybridization analysis of genomic hamster DNA indicates that the sequences present in the clone are tandemly repeated with a redundancy of 2700 copies per diploid cell.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • Cricetinae
  • Cricetulus
  • DNA / metabolism*
  • Female
  • Genes*
  • Kidney
  • Nucleic Acid Hybridization
  • Oocytes / metabolism
  • Plasmids
  • Protein Biosynthesis
  • RNA Polymerase III / metabolism
  • RNA, Ribosomal / genetics*
  • Transcription, Genetic
  • Xenopus

Substances

  • RNA, Ribosomal
  • DNA
  • RNA Polymerase III

Associated data

  • GENBANK/J00063