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The neutral sugars and hexosamines in the seromucoid fraction of normal human serum were identified and determined simultaneously by gas-liquid chromatography of their aldononitrile acetates. Besides the sialic acid, normal seromucoid contained fucose, arabinose, mannose, glucose, galactose, glucosamine and galactosamine. The presence of arabinose and glucose, not previously reported in seromucoid, as well as the other known carbohydrate components determined by GLC were confirmed from their fragmentation patterns in the electron impact mass spectra and chemical ionization spectra.
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