Nucleotide sequence of wheat germ cytoplasmic initiator methionine transfer ribonucleic acid

Nucleic Acids Res. 1982 May 25;10(10):3241-7. doi: 10.1093/nar/10.10.3241.

Abstract

The primary sequence of wheat germ initiator tRNA has been determined using in vitro labelling techniques. The sequence is: pAUCAGAGUm1Gm2GCGCAG CGGAAGCGUm2GG psi GGGCCCAUt6AACCCACAGm7GDm5Cm5CCAGGA psi CGm1AAACCUG*GCUCUGAUACCAOH. As in other eukaryotic initiator tRNAs, the sequence -T psi CG(A)- present in loop IV of virtually all tRNA active in protein synthesis is absent and is replaced by -A psi CG-. The base pair G2:C71 present in all other initiator tRNAs recognized by E. coli Met-tRNA transformylase is absent and is replaced by U2:A71. Since wheat germ initiator tRNA is not formylated by E. coli Met-tRNA transformylase this implies a possible role of the G2:C71 base pair present in other initiator tRNAs in formylation of initiator tRNA species.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cytoplasm / physiology
  • Nucleic Acid Conformation
  • Oligoribonucleotides / analysis
  • Peptide Initiation Factors / genetics*
  • Plants / metabolism*
  • Protein Biosynthesis
  • RNA, Transfer, Amino Acyl / genetics*
  • Ribonuclease T1
  • Ribonucleases
  • Triticum / metabolism

Substances

  • Oligoribonucleotides
  • Peptide Initiation Factors
  • RNA, Transfer, Amino Acyl
  • tRNA(m)(Met), methionine-
  • Ribonucleases
  • Ribonuclease T1

Associated data

  • GENBANK/K00317