Abstract
The guanosine analog 8-aminoguanosine is an effective inhibitor of the purine degradative enzyme purine nucleoside phosphorylase, both in vitro and in intact lymphoid cells. In a human lymphoblast tissue culture system, 8-aminoguanosine, in combination with low concentrations of 2'-deoxyguanosine, causes toxicity toward T cells but not B cells. The selective T cell toxicity correlates with increased accumulation of deoxyguanosine triphosphate in the treated T lymphoblasts.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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B-Lymphocytes / enzymology
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Cell Line
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Deoxyguanosine / pharmacology
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Guanosine / analogs & derivatives*
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Guanosine / pharmacology
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Humans
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Kinetics
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Pentosyltransferases / antagonists & inhibitors*
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Purine-Nucleoside Phosphorylase / antagonists & inhibitors*
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T-Lymphocytes / drug effects
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T-Lymphocytes / enzymology*
Substances
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Guanosine
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8-aminoguanosine
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Pentosyltransferases
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Purine-Nucleoside Phosphorylase
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Deoxyguanosine