Inhibition of purine nucleoside phosphorylase by 8-aminoguanosine: selective toxicity for T lymphoblasts

Science. 1981 Dec 4;214(4525):1137-9. doi: 10.1126/science.6795718.

Abstract

The guanosine analog 8-aminoguanosine is an effective inhibitor of the purine degradative enzyme purine nucleoside phosphorylase, both in vitro and in intact lymphoid cells. In a human lymphoblast tissue culture system, 8-aminoguanosine, in combination with low concentrations of 2'-deoxyguanosine, causes toxicity toward T cells but not B cells. The selective T cell toxicity correlates with increased accumulation of deoxyguanosine triphosphate in the treated T lymphoblasts.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • B-Lymphocytes / enzymology
  • Cell Line
  • Deoxyguanosine / pharmacology
  • Guanosine / analogs & derivatives*
  • Guanosine / pharmacology
  • Humans
  • Kinetics
  • Pentosyltransferases / antagonists & inhibitors*
  • Purine-Nucleoside Phosphorylase / antagonists & inhibitors*
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / enzymology*

Substances

  • Guanosine
  • 8-aminoguanosine
  • Pentosyltransferases
  • Purine-Nucleoside Phosphorylase
  • Deoxyguanosine