J Anal Toxicol. 1984 Mar-Apr;8(2):68-74.

High-performance liquid chromatographic study of codeine, norcodeine, and morphine as indicators of codeine ingestion.

Abstract

A simple quantitative method for determining codeine, norcodeine, and morphine in urine by high-performance liquid chromatography (HPLC) is described. Urine samples are hydrolyzed and extracted through differential pH extraction. The subsequent use of HPLC allowed separation and quantitation on a reverse phase system with ultraviolet detection. A comparison study of the concentrations of single and therapeutic doses of codeine in urine over a period of time showed that morphine, the major metabolite, increases in proportion to codeine, eventually surpasses the codeine level, and remains higher for the duration of time that the drugs are detectable.

PMID:: 6716978; [PubMed - indexed for MEDLINE]

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Medical

Drug Abuse - MedlinePlus Health Information

Molecular Biology Databases

Supplemental Content

Related citations

Determination of codeine and metabolites in plasma and urine using ion-pair high-performance liquid chromatography. [J Chromatogr B Biomed Appl. 1995]
Determination of codeine and metabolites in plasma and urine using ion-pair high-performance liquid chromatography.
Svensson JO, Yue QY, Säwe J. J Chromatogr B Biomed Appl. 1995 Dec 1; 674(1):49-55.
Direct determination of codeine, norcodeine, morphine and normorphine with their corresponding O-glucuronide conjugates by high-performance liquid chromatography with electrochemical detection. [J Chromatogr. 1991]
Direct determination of codeine, norcodeine, morphine and normorphine with their corresponding O-glucuronide conjugates by high-performance liquid chromatography with electrochemical detection.
Verwey-van Wissen CP, Koopman-Kimenai PM, Vree TB. J Chromatogr. 1991 Oct 4; 570(2):309-20.
Simultaneous determination of codeine, norcodeine and morphine in biological fluids by high-performance liquid chromatography with fluorescence detection. [J Chromatogr. 1989]
Simultaneous determination of codeine, norcodeine and morphine in biological fluids by high-performance liquid chromatography with fluorescence detection.
Chen ZR, Bochner F, Somogyi A. J Chromatogr. 1989 Jul 21; 491(2):367-78.
Review High-performance liquid chromatographic determination of morphine in biological samples: an overview of separation methods and detection techniques. [J Chromatogr. 1989]
Review High-performance liquid chromatographic determination of morphine in biological samples: an overview of separation methods and detection techniques.
Tagliaro F, Franchi D, Dorizzi R, Marigo M. J Chromatogr. 1989 Mar 17; 488(1):215-28.
Review [Morphinan alkaloids present in mammal]. [Sheng Li Ke Xue Jin Zhan. 1990]
Review [Morphinan alkaloids present in mammal].
Ning YM, Liu JS. Sheng Li Ke Xue Jin Zhan. 1990 Oct; 21(4):329-32.

See reviews... See all...

Cited by 1 PubMed Central article

Clinical evaluation and use of urine screening for drug abuse. [West J Med. 1988]
Clinical evaluation and use of urine screening for drug abuse.
Saxon AJ, Calsyn DA, Haver VM, Delaney CJ. West J Med. 1988 Sep; 149(3):296-303.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

High-performance liquid chromatographic study of codeine, norcodeine, and morphi...
High-performance liquid chromatographic study of codeine, norcodeine, and morphine as indicators of codeine ingestion.
J Anal Toxicol. 1984 Mar-Apr ;8(2):68-74.

PubMed
The epidemiology of AIDS: current status and future prospects.
The epidemiology of AIDS: current status and future prospects.
Science. 1985 Sep 27 ;229(4720):1352-7.

PubMed
Does HIV cause AIDS? An updated response to Duesberg's theories.
Does HIV cause AIDS? An updated response to Duesberg's theories.
Intervirology. 1990 ;31(6):301-14.

PubMed
Saturnine gout among Roman aristocrats. Did lead poisoning contribute to the fal...
Saturnine gout among Roman aristocrats. Did lead poisoning contribute to the fall of the Empire?
N Engl J Med. 1983 Mar 17 ;308(11):660-3.

PubMed
The incidence of dementia: a meta-analysis.
The incidence of dementia: a meta-analysis.
Neurology. 1998 Sep ;51(3):728-33.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to: