An economic volt-hour integrator, compatible with commercial and homemade electrophoresis power supplies

Anal Biochem. 1984 Nov 1;142(2):515-7. doi: 10.1016/0003-2697(84)90498-6.

Abstract

In isoelectric focusing systems--a technique widely used for separation and characterization of proteins--the value of the volt-hour integral required to achieve steady-state focusing conditions differs markedly for different proteins. A convenient method to measure the correct volt-hour integral for a given protein, thereby assuring intra- and interlaboratory reproducibility of experiments, can be performed with a volt-hour integrator. As commercially available integrators are compatible only with the power supply marketed by the same manufacturer, a volt-hour integrator was designed which can be used in conjunction with any commercial or homemade power supply. This simple, low-cost integrator is expected to be of interest to those researchers who have so far refrained from introducing volt-hour integration, due to incompatibility of commercial volt-hour integrators with their isoelectric focusing system.

MeSH terms

  • Costs and Cost Analysis
  • Electric Power Supplies*
  • Isoelectric Focusing / instrumentation*
  • Time Factors