Abstract
We report the characterization of an amino acid 2-aminoisobutyric acid was transported solely by the general amino acid permease and not by the neutral amino acid permease. Furthermore, this substrate was not metabolized after transport. The potential for a system-specific nonmetabolizable substrate as a tool in the analysis of amino acid transport and its regulation is discussed.
MeSH terms
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Amino Acid Transport Systems
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Aminoisobutyric Acids / metabolism*
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Biological Transport, Active
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Citrulline / metabolism
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Fungal Proteins / biosynthesis
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Kinetics
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Membrane Transport Proteins / metabolism*
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Mutation
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Neurospora / metabolism*
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Neurospora crassa / genetics
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Neurospora crassa / metabolism*
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Substrate Specificity
Substances
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Amino Acid Transport Systems
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Aminoisobutyric Acids
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Fungal Proteins
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Membrane Transport Proteins
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Citrulline