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cDNA cloning of human apoA-I: amino acid sequence of preproapoA-I.
ds-cDNA to human liver apoA-I mRNA has been cloned. Nucleic acid sequence analysis revealed that apoA-I mRNA codes for a precursor apolipoprotein, preproapoA-I, which contains 24 amino acids on the NH2-terminal end of the mature plasma apoA-I. Eighteen amino acids are contained within the hydrophobic prepeptide (Met-Lys-Ala-Ala-Val-Leu-Thr-Leu-Ala-Val-Leu-Phe-Leu-Thr-Gly-Ser-Gln-Ala) followed by a 6 amino acid propeptide (Arg-His-Phe-Trp-Gln-Gln). Our results on human apoA-I are in agreement with the partial sequence of the precursor of rat apoA-I with respect to the length of the precursor sequence, location of the prepeptide, and the presence of an unusual propeptide sequence terminating in a neutral dipeptide Gln-Gln. A detailed analysis of the primary structure of normal human apoA-I mRNA will be essential to our ultimate understanding of the processing of human apoA-I and diseases characterized by molecular defects in apoA-I structure and function.
PMID: 6404278 [PubMed - indexed for MEDLINE]
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Cited by 11 PubMed Central articles
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Human apolipoprotein C-II: complete nucleic acid sequence of preapolipoprotein C-II.
Fojo SS, Law SW, Brewer HB Jr.
Proc Natl Acad Sci U S A. 1984 Oct; 81(20):6354-7.
[Proc Natl Acad Sci U S A. 1984]
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Comparative in vitro study of the pro-apolipoprotein A-I to apolipoprotein A-I converting activity between normal and Tangier plasma.
Edelstein C, Gordon JI, Vergani CA, Catapano AL, Pietrini V, Scanu AM.
J Clin Invest. 1984 Sep; 74(3):1098-103.
[J Clin Invest. 1984]
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Tangier disease: defective recombination of a specific Tangier apolipoprotein A-I isoform (pro-apo A-i) with high density lipoproteins.
Schmitz G, Assmann G, Rall SC Jr, Mahley RW.
Proc Natl Acad Sci U S A. 1983 Oct; 80(19):6081-5.
[Proc Natl Acad Sci U S A. 1983]
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