-
First field trial of an immunoradiometric assay for the detection of malaria sporozoites in mosquitoes.
An immunoradiometric assay (IRMA) using a monoclonal antibody to the major surface protein of Plasmodium falciparum sporozoites was used to assess the P. falciparum sporozoite rate in a West African population of Anopheles gambiae (s.1.). Unlike current dissection techniques, the IRMA could detect sporozoite antigen in dried as well as fresh mosquitoes. In a controlled comparison, the sensitivity of the IRMA was comparable that of the dissection technique. Additionally, the IRMA was species specific and quantitative. Sensitivity of the assay was sufficient to detect sporozoite infections resulting from the development of a single oocyst.
PMID: 6383093 [PubMed - indexed for MEDLINE]
-
Cited by 8 PubMed Central articles
-
Competency of Anopheles stephensi mysorensis strain for Plasmodium vivax and the role of inhibitory carbohydrates to block its sporogonic cycle.
Basseri HR, Doosti S, Akbarzadeh K, Nateghpour M, Whitten MM, Ladoni H.
Malar J. 2008 Jul 15; 7:131. Epub 2008 Jul 15.
[Malar J. 2008]
-
Patterns of selection in anti-malarial immune genes in malaria vectors: evidence for adaptive evolution in LRIM1 in Anopheles arabiensis.
Slotman MA, Parmakelis A, Marshall JC, Awono-Ambene PH, Antonio-Nkondjo C, Simard F, Caccone A, Powell JR.
PLoS One. 2007 Aug 29; 2(8):e793. Epub 2007 Aug 29.
[PLoS One. 2007]
-
Uninfected mosquito bites confer protection against infection with malaria parasites.
Donovan MJ, Messmore AS, Scrafford DA, Sacks DL, Kamhawi S, McDowell MA.
Infect Immun. 2007 May; 75(5):2523-30. Epub 2007 Mar 5.
[Infect Immun. 2007]
- » See all...