Molecular analysis of the recF gene of Escherichia coli

Proc Natl Acad Sci U S A. 1984 Aug;81(15):4622-6. doi: 10.1073/pnas.81.15.4622.

Abstract

We analyzed the nucleotide sequence of a 1.325-kilobase region of wild-type Escherichia coli containing a functional recF gene and six Tn3 mutations that inactivate recF. The analysis shows a potentially translatable reading frame of 1071 nucleotides, which is interrupted by all six insertions. A protein of 40.5 kilodaltons would result from translation of the open reading frame, and a radioactive band of protein of an apparent molecular weight of approximately 40 kilodaltons was seen by the maxicell method using a recF+ plasmid. Putative truncated peptides were seen when two recF::Tn3 mutant plasmids were used. Differential expression of dnaN and recF from a common promoter was noted. recF332::Tn3 was transferred to the chromosome where, in hemizygous condition, it produced UV sensitivity indistinguishable from that produced by two presumed recF point mutations.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Base Sequence
  • DNA Repair
  • DNA Replication
  • Escherichia coli / genetics*
  • Genes, Bacterial*
  • Molecular Weight
  • Mutation
  • Plasmids
  • Recombination, Genetic

Substances

  • Bacterial Proteins

Associated data

  • GENBANK/K02179