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J Bacteriol. 1984 Mar;157(3):712-7.

Identification of the purC gene product of Escherichia coli.

Abstract

The purC region of the Escherichia coli chromosome was isolated from in vivo-derived lambda transducing bacteriophages and cloned in high-copy-number plasmids. The product of the purC gene, phosphoribosylaminoimidazolesuccinocarboxamide synthetase, was identified as a protein with an Mr of ca. 27,000. The level of the protein is increased by more than 60-fold in strains carrying the gene on a high-copy-number plasmid. Purine addition represses the enzyme level in both plasmid- and non-plasmid-containing strains.

PMID:
6365889
[PubMed - indexed for MEDLINE]
PMCID:
PMC215316
Free PMC Article
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