Mutation in the a block of the yeast tRNAleu3 gene that allows transcription but abolishes splicing and 5'-end maturation

Cell. 1983 Jan;32(1):67-76. doi: 10.1016/0092-8674(83)90497-x.

Abstract

A three-base substitution mutant of the yeast tRNALeu3 has been constructed. The mutation, introduced through the use of a heptadecanucleotide as a site-specific mutagen, is localized in the anterior portion of the promoter and results in the inability to form a D stem. The mutant is active in transcription, but maturation of the 5' terminus and splicing are abolished. The results are discussed in the light of a recently proposed model for initiation of transcription of eucaryotic tRNA genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Genes
  • Leucine
  • Mutation
  • Nucleic Acid Precursors / metabolism
  • Plasmids
  • RNA Processing, Post-Transcriptional*
  • RNA, Transfer / genetics*
  • Saccharomyces cerevisiae / genetics*
  • Transcription, Genetic*

Substances

  • Nucleic Acid Precursors
  • RNA, Transfer
  • Leucine

Associated data

  • GENBANK/K01973
  • GENBANK/K02005