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J Mol Biol. 1984 Jun 15;176(1):155-9.

In vitro replication of a dam methylated and non-methylated ori-C plasmid.

Abstract

We have examined the replication of a dam methylated and non-methylated ori-C plasmid in an in vitro ori-C dependent replication system. The results show that the non-methylated plasmid is 50% to 80% less efficient in the initiation of DNA synthesis; that the methylation state of the plasmid does not change the site of initiation at ori-C, and that in both cases initiation at this region requires the presence of exogenously furnished dnaA protein.

PMID:
6330369
[PubMed - indexed for MEDLINE]
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