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    Mol Cell Biol. 1984 May;4(5):994-6.

    Toxicity of 2-deoxygalactose to Saccharomyces cerevisiae cells constitutively synthesizing galactose-metabolizing enzymes.

    Platt T.

    Analysis of 400 independent spontaneous mutations conferring 2-deoxygalactose resistance upon cells constitutive for the galactose pathway suggests that toxicity is due to 2-deoxygalactose-1-phosphate. Selection for and against growth on galactose in the same strain is now possible; application to systems with transcriptional or translational gene fusions to galactokinase are discussed.

    PMID: 6328283 [PubMed - indexed for MEDLINE]

    PMCID: PMC368858

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