Format

Send to:

Choose Destination
See comment in PubMed Commons below
EMBO J. 1982;1(12):1573-8.

Recombinant retroviral DNA yielding high expression of hepatitis B surface antigen.

Abstract

A genomic fragment of hepatitis B virus encoding the surface antigen (HBsAg) was inserted into the proviral genome of Moloney mouse sarcoma virus (MSV), obtained from the mouse cell line G8 -124. The recombinant DNA was introduced into NIH 3T3 mouse fibroblasts. Cells, morphologically transformed by the oncogene of MSV (v-mosM) were selected, established as cell lines and tested for expression of HBsAg. An expression level of up to 4.5 micrograms/10(7) cells/day was detected.

PMID:
6327280
[PubMed - indexed for MEDLINE]
PMCID:
PMC553253
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for PubMed Central
    Loading ...
    Write to the Help Desk