Nucleic Acids Res. 1984 Feb 10;12(3):1671-86.

2',3'-Dideoxy-3' aminonucleoside 5'-triphosphates are the terminators of DNA synthesis catalyzed by DNA polymerases.

Chidgeavadze ZG, Beabealashvilli RS, Atrazhev AM, Kukhanova MK, Azhayev AV, Krayevsky AA.

Abstract

It is shown that 2',3'-dideoxy-3'-aminonucleoside 5'-triphosphates with adenine, guanine, cytosine and thymine bases are effective inhibitors of DNA polymerase I, calf thymus DNA polymerase alpha and rat liver DNA polymerase beta. The effect of the above-mentioned compounds is markedly higher than corresponding action of the well-known DNA synthesis inhibitors arabinonucleoside 5'-triphosphates and 2',3'-dideoxynucleoside 5'-triphosphates. 2',3'-dideoxy-3'-aminonucleoside 5'-monophosphate residues incorporate into the 3'-terminus of the primer and terminate the DNA chain elongation. The possibility of using 2',3'-dideoxy-3'-aminonucleoside 5'-triphosphates as terminators for DNA sequencing by the polymerization method is demonstrated.

PMID:: 6322115; [PubMed - indexed for MEDLINE]
PMCID:: PMC318607

Free PMC Article

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

3'-Hydroxymethyl 2'-deoxynucleoside 5'-triphosphates are inhibitors highly specific for reverse transcriptase. [FEBS Lett. 1986]
3'-Hydroxymethyl 2'-deoxynucleoside 5'-triphosphates are inhibitors highly specific for reverse transcriptase.
Kutateladze TV, Kritzyn AM, Florentjev VL, Kavsan VM, Chidgeavadze ZG, Beabealashvilli RS. FEBS Lett. 1986 Oct 27; 207(2):205-12.
2',3'-Dideoxy-3'-aminonucleoside 5'-triphosphates inhibit the repair of DNA in rat liver chromatin. [Biochim Biophys Acta. 1984]
2',3'-Dideoxy-3'-aminonucleoside 5'-triphosphates inhibit the repair of DNA in rat liver chromatin.
Krayevsky A, Kukhanova M, Alexandrova L, Belyakova N, Krutyakov V. Biochim Biophys Acta. 1984 Dec 14; 783(3):216-20.
3'-Fluoro-2',3'-dideoxyribonucleoside 5'-triphosphates: terminators of DNA synthesis. [FEBS Lett. 1985]
3'-Fluoro-2',3'-dideoxyribonucleoside 5'-triphosphates: terminators of DNA synthesis.
Chidgeavadze ZG, Scamrov AV, Beabealashvilli RS, Kvasyuk EI, Zaitseva GV, Mikhailopulo IA, Kowollik G, Langen P. FEBS Lett. 1985 Apr 22; 183(2):275-8.
Review Nucleotide probes of DNA polymerases. [Acta Biochim Pol. 1996]
Review Nucleotide probes of DNA polymerases.
Wright GE. Acta Biochim Pol. 1996; 43(1):115-24.
Review The mechanism of action of inhibitors of DNA synthesis. [Annu Rev Biochem. 1977]
Review The mechanism of action of inhibitors of DNA synthesis.
Cozzarelli NR. Annu Rev Biochem. 1977; 46:641-68.

See reviews... See all...

Cited by 4 PubMed Central articles

Design and synthesis of a 3'-O-allyl photocleavable fluorescent nucleotide as a reversible terminator for DNA sequencing by synthesis. [Proc Natl Acad Sci U S A. 2005]
Design and synthesis of a 3'-O-allyl photocleavable fluorescent nucleotide as a reversible terminator for DNA sequencing by synthesis.
Ruparel H, Bi L, Li Z, Bai X, Kim DH, Turro NJ, Ju J. Proc Natl Acad Sci U S A. 2005 Apr 26; 102(17):5932-7. Epub 2005 Apr 13.
Termination of DNA synthesis by novel 3'-modified-deoxyribonucleoside 5'-triphosphates. [Nucleic Acids Res. 1994]
Termination of DNA synthesis by novel 3'-modified-deoxyribonucleoside 5'-triphosphates.
Metzker ML, Raghavachari R, Richards S, Jacutin SE, Civitello A, Burgess K, Gibbs RA. Nucleic Acids Res. 1994 Oct 11; 22(20):4259-67.
Catalytic editing properties of DNA polymerases. [Proc Natl Acad Sci U S A. 1995]
Catalytic editing properties of DNA polymerases.
Canard B, Cardona B, Sarfati RS. Proc Natl Acad Sci U S A. 1995 Nov 21; 92(24):10859-63.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

2',3'-Dideoxy-3' aminonucleoside 5'-triphosphates are the terminators of DNA syn...
2',3'-Dideoxy-3' aminonucleoside 5'-triphosphates are the terminators of DNA synthesis catalyzed by DNA polymerases.
Nucleic Acids Res. 1984 Feb 10 ;12(3):1671-86.

PubMed
Sensory neuropathy from megadoses of pyridoxine.
Sensory neuropathy from megadoses of pyridoxine.
Nutr Rev. 1984 Feb ;42(2):49-51.

PubMed
The Na,K-ATPase hypothesis for manic-depression. I. General considerations.
The Na,K-ATPase hypothesis for manic-depression. I. General considerations.
Med Hypotheses. 1983 Nov ;12(3):253-68.

PubMed
Endogenous xenotropic murine leukemia virus-related sequences map to chromosomal...
Endogenous xenotropic murine leukemia virus-related sequences map to chromosomal regions encoding mouse lymphocyte antigens.
J Virol. 1984 Apr ;50(1):237-47.

PubMed
Canine parvovirus immunoprophylaxis: a review.
Canine parvovirus immunoprophylaxis: a review.
J S Afr Vet Assoc. 1983 Dec ;54(4):259-64.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

2',3'-Dideoxy-3' aminonucleoside 5'-triphosphates are the terminators of DNA synthesis catalyzed by DNA polymerases.

Abstract

Publication Types, MeSH Terms, Substances, Secondary Source ID

Publication Types

MeSH Terms

Substances

Secondary Source ID

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Cited by 4 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information