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Nature. 1984 Mar 1-7;308(5954):32-6.

Cryo-electron microscopy of viruses.

Abstract

Thin vitrified layers of unfixed, unstained and unsupported virus suspensions can be prepared for observation by cryo-electron microscopy in easily controlled conditions. The viral particles appear free from the kind of damage caused by dehydration, freezing or adsorption to a support that is encountered in preparing biological samples for conventional electron microscopy. Cryo-electron microscopy of vitrified specimens offers possibilities for high resolution observations that compare favourably with any other electron microscopical method.

PMID:
6322001
[PubMed - indexed for MEDLINE]
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