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Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli.
A plasmid conferring resistance to the aminocyclitol antibiotic hygromycin B was isolated from Escherichia coli. The gene conferring resistance to this drug was cloned in pBR322, and the gene was localized to a fragment of ca. 1,510 base pairs. Resistance to hygromycin B is determined by an aminocyclitol phosphotransferase that modifies hygromycin B and structurally related antibiotics. The specific modification of hygromycin B is a phosphorylation of the hydroxyl on the 4 position of the cyclitol ring (hyosamine). The presence of the phosphotransferase in E. coli correlates with reduced accumulation of [14C]hygromycin B.
PMID: 6318654 [PubMed - indexed for MEDLINE]
PMCID: PMC185926
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Cited by 10 PubMed Central articles
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Structural basis for hygromycin B inhibition of protein biosynthesis.
Borovinskaya MA, Shoji S, Fredrick K, Cate JH.
RNA. 2008 Aug; 14(8):1590-9. Epub 2008 Jun 20.
[RNA. 2008]
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Role of 16S rRNA Helix 44 in Ribosomal Resistance to Hygromycin B.
Pfister P, Risch M, Brodersen DE, Böttger EC.
Antimicrob Agents Chemother. 2003 May; 47(5):1496-502.
[Antimicrob Agents Chemother. 2003]
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The bldD gene of Streptomyces coelicolor A3(2): a regulatory gene involved in morphogenesis and antibiotic production.
Elliot M, Damji F, Passantino R, Chater K, Leskiw B.
J Bacteriol. 1998 Mar; 180(6):1549-55.
[J Bacteriol. 1998]
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