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1: Antimicrob Agents Chemother. 1983 Nov;24(5):689-95.Click here to read Click here to read Links

Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli.

Rao RN, Allen NE, Hobbs JN Jr, Alborn WE Jr, Kirst HA, Paschal JW.

A plasmid conferring resistance to the aminocyclitol antibiotic hygromycin B was isolated from Escherichia coli. The gene conferring resistance to this drug was cloned in pBR322, and the gene was localized to a fragment of ca. 1,510 base pairs. Resistance to hygromycin B is determined by an aminocyclitol phosphotransferase that modifies hygromycin B and structurally related antibiotics. The specific modification of hygromycin B is a phosphorylation of the hydroxyl on the 4 position of the cyclitol ring (hyosamine). The presence of the phosphotransferase in E. coli correlates with reduced accumulation of [14C]hygromycin B.

PMID: 6318654 [PubMed - indexed for MEDLINE]

PMCID: PMC185926

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