Abstract
Mitochondria isolated from Neurospora crassa were purified by centrifugation in a Percoll density gradient. Enzyme activities and cytochrome differential spectra revealed a high purity of the mitochondria. As compared with a crude mitochondrial fraction the purified mitochondria exhibited a high respiratory activity and a fine ADP/O ratio. Electrophoresis of nucleic acids demonstrated the absence of cytoplasmic rRNA.
MeSH terms
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Adenosine Diphosphate / analysis
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Centrifugation, Density Gradient
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Cytochromes / analysis
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Electron Transport Complex IV / analysis
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L-Lactate Dehydrogenase / analysis
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Mitochondria / analysis*
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Mitochondria / enzymology
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Neurospora / ultrastructure*
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Neurospora crassa / ultrastructure*
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RNA, Fungal / analysis
Substances
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Cytochromes
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RNA, Fungal
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Adenosine Diphosphate
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L-Lactate Dehydrogenase
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Electron Transport Complex IV