Biochem Biophys Res Commun. 1983 Aug 12;114(3):955-61.

Activation of calcineurin by nickel ions.

Abstract

Calcineurin, a Ca2+- and calmodulin-dependent phosphoprotein phosphatase, was dramatically activated by Ni2+ ions. Activation by Ni2+ was independent of calmodulin and was not reversed by high concentrations of chelators. With histone H1 as substrate, the Km's obtained with Ca2+ and Ni2+ were 2.2 and 4.2 microM, and the kcat's were 0.5 and 24.3 min-1, respectively. Similar to the Ca2+- and Mn2+-supported reactions, the presence of calmodulin caused a 20-fold activation of the Ni2+-activated calcineurin over the basal rate. Incubation of calcineurin with Ni2+ resulted in 30% quenching of its Trp-fluorescence. This effect also was independent of calmodulin and not reversed by chelators. The results suggest that the Ni2+ ions are tightly bound to calcineurin and the effects may be physiologically relevant.

PMID:: 6311199; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Elsevier Science

Molecular Biology Databases

Supplemental Content

Save items

Related citations in PubMed

Divalent cation effects on calcineurin phosphatase: differential involvement of hydrophobic and metal binding domains in the regulation of the enzyme activity. [Mol Cell Biochem. 1990]
Divalent cation effects on calcineurin phosphatase: differential involvement of hydrophobic and metal binding domains in the regulation of the enzyme activity.
Gupta RC, Khandelwal RL, Sulakhe PV. Mol Cell Biochem. 1990 Sep 3; 97(1):53-66.
Regulation of calcineurin by metal ions. Mechanism of activation by Ni2+ and an enhanced response to Ca2+/calmodulin. [J Biol Chem. 1984]
Regulation of calcineurin by metal ions. Mechanism of activation by Ni2+ and an enhanced response to Ca2+/calmodulin.
Pallen CJ, Wang JH. J Biol Chem. 1984 May 25; 259(10):6134-41.
The calmodulin-dependent activation and deactivation of the phosphoprotein phosphatase, calcineurin, and the effect of nucleotides, pyrophosphate, and divalent metal ions. Identification of calcineurin as a Zn and Fe metalloenzyme. [J Biol Chem. 1984]
The calmodulin-dependent activation and deactivation of the phosphoprotein phosphatase, calcineurin, and the effect of nucleotides, pyrophosphate, and divalent metal ions. Identification of calcineurin as a Zn and Fe metalloenzyme.
King MM, Huang CY. J Biol Chem. 1984 Jul 25; 259(14):8847-56.
Review A multifunctional calmodulin-stimulated phosphatase. [Arch Biochem Biophys. 1985]
Review A multifunctional calmodulin-stimulated phosphatase.
Pallen CJ, Wang JH. Arch Biochem Biophys. 1985 Mar; 237(2):281-91.
Review Calcineurin: not just a simple protein phosphatase. [Biochem Biophys Res Commun. 1997]
Review Calcineurin: not just a simple protein phosphatase.
Guerini D. Biochem Biophys Res Commun. 1997 Jun 18; 235(2):271-5.

See reviews... See all...

Cited by 2 PubMed Central articles

Insulin-receptor phosphotyrosyl-protein phosphatases. [Biochem J. 1988]
Insulin-receptor phosphotyrosyl-protein phosphatases.
King MJ, Sale GJ. Biochem J. 1988 Dec 15; 256(3):893-902.
Characterization of a cDNA clone encoding the calmodulin-binding domain of mouse brain calcineurin. [Proc Natl Acad Sci U S A. 1988]
Characterization of a cDNA clone encoding the calmodulin-binding domain of mouse brain calcineurin.
Kincaid RL, Nightingale MS, Martin BM. Proc Natl Acad Sci U S A. 1988 Dec; 85(23):8983-7.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound
PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance
PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Activation of calcineurin by nickel ions.
Activation of calcineurin by nickel ions.
Biochem Biophys Res Commun. 1983 Aug 12 ;114(3):955-61.

PubMed
On the mechanism by which antiadrenergic drugs increase survival of critical ski...
On the mechanism by which antiadrenergic drugs increase survival of critical skin flaps.
Plast Reconstr Surg. 1983 Oct ;72(4):518-25.

PubMed
Construction of high copy yeast vectors using 2-microns circle sequences.
Construction of high copy yeast vectors using 2-microns circle sequences.
Methods Enzymol. 1983 ;101:307-25.

PubMed
Brain stimulation in the study of neuronal functions for conscious sensory exper...
Brain stimulation in the study of neuronal functions for conscious sensory experiences.
Hum Neurobiol. 1982 ;1(4):235-42.

PubMed
Myocardial imaging in myocardial infarction: technetium vs. thallium.
Myocardial imaging in myocardial infarction: technetium vs. thallium.
Cardiovasc Clin. 1983 ;13(3):223-42.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Activation of calcineurin by nickel ions.

Abstract

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Supplemental Content

Save items

Related citations in PubMed

Cited by 2 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information