The structural organization and DNA sequence of a wheat histone H4 gene

Nucleic Acids Res. 1983 Sep 10;11(17):5865-75. doi: 10.1093/nar/11.17.5865.

Abstract

Some wheat histone H4 genes have been cloned from a Charon 4 wheat genomic DNA library using sea urchin histone H4 DNA as a probe. DNA sequence analysis of a cloned gene showed that the deduced amino acid sequence of wheat histone H4 protein was identical to that of pea. The 5' end of wheat histone H4 mRNA was mapped on the cloned gene by the S1-procedure. Southern blotting analysis of the genomic DNA indicated that histone H4 genes were reiterated 100 to 125 times per hexaploid wheat genome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics*
  • DNA Restriction Enzymes
  • Genes*
  • Histones / genetics*
  • Nucleic Acid Hybridization
  • Plants / genetics*
  • Sea Urchins
  • Triticum / genetics

Substances

  • Histones
  • DNA
  • DNA Restriction Enzymes

Associated data

  • GENBANK/X00043